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Product category
Human cells
Organism
Homo sapiens, human
Morphology
epithelial
Tissue
Uterus; Cervix
Disease
Adenocarcinoma
Applications
3D cell culture
Product format
Frozen
General
Specific applications
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al.
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
Characteristics
Growth properties
Adherent
Derivation
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).
Age
31 years
Ethnicity
Black
Gender
Female
Genes expressed
keratin
Isoenzymes
G6PD, A
Comments
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
The line is usually carried as monolayer cultures in Leibovitz's L-15 medium, but can be readily adapted to other media and to suspension culture (in media without Ca++).
The H-HeLa line was cured of a mycoplasma contamination by W.M. Lee in Rueckert's laboratory and the cured line was given the designation H1-HeLa.
Handling information
Unpacking and storage instructions
Check all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Temperature
37°C
Atmosphere
100% Air
Handling procedure
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 7 minutes. Discard supernatant.
Resuspend the cell pellet with the recommended complete medium and dispense into a culture flask. 
Incubate the culture at 37°C in a suitable incubator in a free gas exchange with atmospheric air
Subculturing procedure
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove the medium and add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the culture to sit for 5 minutes at room temperature. Add fresh culture medium, aspirate and dispense into new flasks.
Quality control specifications
Mycoplasma contamination
Not detected
Virus testing
Human papillomavirus (HPV): Detected
STR profiling
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
TH01: 7
TPOX: 8,12
vWA: 16,18
D3S1358: 15,18
D21S11: 27,28
D18S51: 16
Penta_E: 7,17,18
Penta_D: 8,15
D8S1179: 12,13
FGA: 18,21
D19S433: 13,14
D2S1338: 17
History
Deposited as
Homo sapiens
Depositors
RR Rueckert
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.