办公室疯狂高潮呻吟视频,又黄又大又爽又刺激的免费视频,激情综合一区二区三区,青草久久久国产线免费

IM-9

CCL-159 ™

EXPLORE OUR DATA

Product category

Human cells

Organism

Homo sapiens, human

Cell type

B lymphoblast

Morphology

lymphoblast

Tissue

Peripheral blood

Disease

Multiple Myeloma

Applications

3D cell culture

Immunology

Product format

Frozen

Storage conditions

Vapor phase of liquid nitrogen

Characteristics

Growth properties

Suspension

Derivation

Although derived from the blood of a patient with multiple myeloma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line.

Ethnicity

White

Gender

Female

Karyotype

normal human female; diploid; stable; Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

Antigen expression

CD11a +; CD19 +; CD20 +; CD38 -; CD49e +

Genes expressed

immunoglobulin

Expression markers

Growth hormone receptor; insulin receptor; calcitonin receptor

Isoenzymes

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 2

PGM1, 1-2

PGM3, 0

Isotype

IgG, kappa light chain

Comments

The cells are EBNA positive. 

ATCC confirmed this cell line is positive for the presence of Epstein-Barr (EBV) viral DNA sequences via PCR.

Handling information

Unpacking and storage instructions

Check all containers for leakage or breakage.

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Temperature

37°C

Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 10 minutes.

Resuspend the cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 or a 75 cm2 culture flask.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product.

Subculturing procedure

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1x 105 and 1 x 106 viable cells/mL. Do not allow the cell density to exceed 2 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Reagents for cryopreservation

Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)

Quality control specifications

Mycoplasma contamination

Not detected

Virus testing

Epstein-Barr virus (EBV): Detected

STR profiling

Amelogenin: X

CSF1PO: 10,11

D13S317: 9,11

D16S539: 9,13

D5S818: 13

D7S820: 11,12

TH01: 6,9.3

TPOX: 11

vWA: 14,17

D3S1358: 14,18

D21S11: 30,30.2

D18S51: 14,17

Penta_E: 13,15

Penta_D: 9,11

D8S1179: 12,13

FGA: 20,23

D19S433: 13,14

D2S1338: 24,26

History

Deposited as

Homo sapiens

Depositors

DN Buell

Year of origin

1967

Cross references

GenBank X81121 H.sapiens mRNA for central cannabinoid receptor, short isoform.

GenBank X84369 H.sapiens RNA for heavy chain variable and constant regions (IM9-VH).

GenBank U07989 Human Burkitt's lymphoma immunoglobulin kappa light chain mRNA, partial cds.

GenBank U07985 Human myeloma immunoglobulin heavy chain Fd region V-D-J-CH1 mRNA, partial cds.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.