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ATCC? Number:PCS-200-013
Name: Primary Epidermal Melanoyctes; Normal, Human, Adult
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Foreskin, lightly pigmented Epidermis (basal layer)
Growth Properties: Adherent
Morphology: Stellar, multipolar or dendritic apperance; needle-like
Number of Viable Cells per Vial: ≥ 500,000 in cryopreservation medium
Volume per Vial: 1 ml
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Dermal Cell Basal Medium (ATCCPCS-200-030)
Adult Melanocyte Growth Kit (ATCCPCS-200-042)
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC30-2200)
Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)
Trypsin Neutralizing Solution (ATCCPCS-999-004)
Gentamicin-Amphotericin B Solution (ATCCPCS-999-025)
Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)
Phenol Red (ATCCPCS-999-001)

Comments:ATCC? Normal Human Primary Epidermal Melanocytes from from adult tissue, when grown in Dermal Cell Basal Media supplemented with Adult Melanocyte Growth Kit components, provide an ideal cell system to propagate adult melanocytes in low serum (less than 1.0% FBS) conditions in the absence of cholera toxin and phorbol 12-myristate 13-acetate (PMA). The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency.
Applications:Melanoma, response to UV radiation, psoriasis and other skin diseases, skin trauma (e.g., wound repair, scars, burns), cosmetic research (e.g., skin lightening compounds, skin protecting compounds)
Viability:≥ 50%
Population Doubling Capacity:≥ 10 in complete growth medium
Sterility Tests:Bacteria and Yeast: Negative Mycoplasma: Negative
Testing:Hepatitis B: Negative Hepatitis C: Negative HIV-1 and HIV-2 Testing: Negative