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生長狀態(tài)：貼壁生長 

ATCC Number：TIB-71

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運輸方式：凍存運輸 

品系：BALB/c 

組織來源：ascites 

細(xì)胞類型：其他細(xì)胞類型 

是否是腫瘤細(xì)胞：0 

物種來源：小鼠 

年限：adult 

數(shù)量：大量 

細(xì)胞形態(tài)：單核細(xì)胞/巨噬細(xì)胞 

規(guī)格：0.5ml                                                                                                                 Designations 統(tǒng)一別名 : RAW 264.7         

Depositors:WC Raschke         

Biosafety Level 生物安全等級 :2         

Shipped 提供形式 :frozen         

Medium & Serum: See Propagation

Growth Properties:adherent   

Organism:Mus musculus 

Morphology:monocyte/macrophage    

Source:  

Strain: BALB/c    

Tissue: ascites         

Disease: Abelson murine leukemia virus-induced tumor 

Cell Type:macrophage; Abelson murine leukemia virus transformed    

Cellular Products:lysozyme 

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits  may be required for the          transfer of this ATCC material. 

Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.

Please click here for information regarding the specific requirements for shipment to your location.

Applications 用途 :Biological response transfection host    

Receptors:complement (C3)  [1207 ] Antigen Expression:H-2d 

Age:adult     

Gender:male     

Comments 注釋 :This line was established from a tumor induced by Abelson murine leukemia virus. They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2) This line does not secrete detectable virus particles and is negative in the XC plaque formation assay.

The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets.

LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets. Data communicated in Feb. 2007 by Dr Janet W. Hartley, indicates the expression of infectious ecotropic MuLV closely related, if not identical,to the Moloney MuLV helper virus used in the original virus inoculum. The cells also express polytropic MuLV,unsurprisingly based on the mouse passage history of the virus stocks [ PubMed 18177500]. 

Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.  

To make the complete growth medium, add the following components to the base medium:  fetal bovine serum to a final concentration of 10%.

Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2),5%         

Temperature 培養(yǎng)溫度 : 37.0°C         

Subculturing: 

Protocol: Subcultures are prepared by scraping. 

For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels).  

Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels. 

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended    

Medium Renewal: Replace or add medium every 2 to 3 days. 

Preservation:

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO    

Storage temperature 保藏溫度 : liquid nitrogen vapor phase

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020         

References 參考文獻(xiàn) :135: Ralph P, Nakoinz I.Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.J.Immunol. 119: 950-954, 1977. PubMed: 894031

1207: Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus.Cell 15: 261-267, 1978. PubMed: 212198          

32443: Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium.J. Biol. Chem. 271: 337-342,1996. PubMed: 8550583

32466: Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages.  Proc. Natl. Acad.Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

32553: Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma.J. Biol. Chem.271: 20399-20405, 1996. PubMed: 8702776

32901: Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins.Proc. Natl. Acad. Sci. USA 93:11047-11052, 1996. PubMed: 8855306

33046: Panneerselvam K, Freeze HH.Mannose enters mammalian cells using a specific transporter that is insensitive toglucose.J. Biol. Chem. 271:9417-9421, 1996. PubMed: 8621609

33076: Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus.  J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857

33162: Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA.  J. Biol. Chem. 271:17445-17452, 1996. PubMed: 8663413

92560:Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken,PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

16173094: Hartley JW, et al.Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008.PubMed 18177500.