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細胞形態(tài)：淋巴樣
年限：5 years
ATCC Number：CRL-8885?
相關(guān)疾?。浩渌膊?br /> 生長狀態(tài)：懸浮生長
器官來源：脾
數(shù)量：大量
細胞類型：其他細胞類型
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：人
規(guī)格：0.2ml Designations: WIL2-S
Depositors: ?Lovelace Medical Foundation
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast


Source: Organ: spleen
Disease: hereditary spherocytosis
Cell Type: lymphoblast B lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
F13A01: 6,7
F13B: 10
FESFPS: 11,12
LPL: 9,10
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Age: 5 years
Gender: male
Ethnicity: Caucasian
Comments:This is a HAT sensitive variant of the WIL2 human B cell line (see also ATCC CRL-8155 ).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:WIL2-NS, ATCC CRL-8155
TK6, ATCC CRL-8015
LTR228 , ATCC HB-8502