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年限：56 years
是否是腫瘤細胞：1
物種來源：人
生長狀態(tài)：貼壁生長
細胞形態(tài)：上皮樣
ATCC Number：HTB-43?
器官來源：咽
相關(guān)疾?。瑚[狀細胞癌
運輸方式：凍存運輸
數(shù)量：大量
規(guī)格：0.1ml Designations: FaDu
Depositors: ?SR Rangan
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: pharynx
Disease: squamous cell carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: None detected
CSF1PO: 12
D13S317: 8, 9
D16S539: 11
D5S818: 12
D7S820: 11, 12
THO1: 8
TPOX: 11
vWA: 15, 17, 18
Cytogenetic Analysis:(P16) hypodiploid to hypertriploid with modal number = 64
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 2
PGM3, 1
Age: 56 years
Gender: male
Ethnicity: Caucasian
Comments:The FaDu line was established in 1968 from a punch biopsy of an hypopharyngeal tumor removed from a Hindu patient.
The established line was found to contain bundles of tonofilaments in the cell cytoplasm and desmosomal regions were prominent at cell boundaries.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22792: Rangan SR. A new human cell line (FaDu) from a hypopharyngeal carcinoma. Cancer 29: 117-121, 1972. PubMed: 4332311
23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758