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生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：CRL-1628?
相關(guān)疾?。瑚[狀細(xì)胞癌
數(shù)量：大量
年限：70 years
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：舌頭
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
規(guī)格：100次 Designations: SCC-25
Depositors: ?JG Rheinwald
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: tongue
Disease: squamous cell carcinoma
Cellular Products:epidermal keratins; low levels of involucrin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 10
D13S317: 13
D16S539: 11,12
D5S818: 12
D7S820: 12
THO1: 8
TPOX: 8,12
vWA: 17,19
Age: 70 years
Gender: male
Comments:SCC-25 cells are capable of only limited proliferation in semi-solid medium.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate and supplemetned with 400 ng/ml hydrocortisone and 10% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Cells are disaggregated to single cells when the culture is preconfluent. They do not require a feeder layer.
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336