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生長狀態(tài)：貼壁生長
器官來源：大腦
年限：classified as grade IV as of 2007
數(shù)量：大量
細胞形態(tài)：上皮樣
ATCC Number：HTB-14?
運輸方式：凍存運輸
相關(guān)疾?。浩渌膊?br /> 是否是腫瘤細胞：1
物種來源：人
規(guī)格：10 μg Designations: U-87 MG
Depositors: ?J Ponten
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: brain
Tumor Stage: classified as grade IV as of 2007
Disease: glioblastoma; astrocytoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Tumorigenic:Yes
Antigen Expression:Blood Type A, Rh+
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D7S820: 8,9
D5S818: 11,12
D16S539: 12
vWA: 15,17
THO1: 9.3
TPOX: 8
Cytogenetic Analysis:This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1
PGM1, 2
PGM3, 1
Age: 44 years
Gender: male
Ethnicity: Caucasian
Comments:This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-15 and ATCC HTB-16 ) by J. Ponten and associates from 1966 to 1969.
Mycoplasma contamination was eliminated in September 1975.
Note: ATCC has confirmed that the ATCC ^? HTB-14? cell line is male in origin based on STR, Y-chromosome paint and Q-band assays. However, based on current literature, the cell line is still of glioblastoma origin and the discrepancy of gender is not unusual. It is possible that the cell line was misidentified in the depositor's original publication.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

• Remove and discard culture medium.
• Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
• Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
• Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
• Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
• Incubate cultures at 37C.

Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22159: Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
23128: Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504
32901: Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
16173472: Clark MJ, et al. U87MG decoded: The genomic sequence of a cytogenetically aberrant human cancer cell line. PLoS Genetics 6 (1) : e1000832, 2010.