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數(shù)量：大量 

生長狀態(tài)：懸浮生長，多細(xì)胞聚集

 細(xì)胞形態(tài)：其他 

年限：55 years adult 

ATCC Number：HTB-119

 相關(guān)疾病：小細(xì)胞肺癌 

是否是腫瘤細(xì)胞：1 

物種來源：人

 運(yùn)輸方式：凍存運(yùn)輸 

器官來源：肺 

規(guī)格：0.1ml

 NCI-H69 [H69] (ATCC? HTB-119?)

Organism Homo sapiens, human Tissue lung 

Product Format frozen 

Morphology floating aggregates

 Culture Properties suspension, multicell aggregates 

Biosafety Level 1 

 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Disease carcinoma; small cell lung cancer 

Age 55 years adult Gender male

 Ethnicity Caucasian 

Storage Conditions liquid nitrogen vapor temperature

Karyotype modal number = 76 to 78; range = 40 to 87 This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der(16)t(1;16)(q21;q23), der(22)t(4;22)(q12;q13), der(12)t(11;12)(q23;p12), del(17)(p11), der(19)t(5;19)(?q21;q13) and others. 

Clinical Data 55 years adult Caucasian male 

Receptor Expression insulin-like growth factor II (IGF II) Oncogene myc +; myb +; fes +; fms +; raf +; ras + 

Genes Expressed Oncogenes: myc +; myb +; fes +; fms +; raf +; ras + Tumorigenic Yes

Effects Yes, in nude mice (The cells form tumors with typical small cell carcinoma histology.) Yes, forms colonies in soft agar 

Comments This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. The cells grow in aggregates, thus cell counts are not accurate. The cells stain positively for cytokeratins. The line can be adapted to grow in shaker flask or spinner flask systems. The N-myc gene is amplified, and there is expression of the mRNA and protein. C-myc mRNA, but not protein, is expressed at a low level. There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 times weekly Cryopreservation Freeze medium:

 Complete growth medium, 95%; DMSO, 5% 

Storage temperature: liquid nitrogen vapor temperature

 Culture Conditions

 Temperature: 37.0°C

 STR Profile CSF1PO: 10, 12; D13S317: 12; D16S539: 11; D5S818: 11, 13 ;D7S820: 9; THO1: 8, 9; TPOX: 10; vWA: 16, 17 ;Amelogenin: XY

 Isoenzymes AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 2 PGM3, 1