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年限：7
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：kappa light chain
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)，多細(xì)胞聚集
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-1484?
相關(guān)疾?。毫馨土? Designations: HS-Sultan
Depositors: ?NS Harris
Isotype: kappa light chain
Biosafety Level:2 [Cells contain EBV DNA viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, multicell aggregates
Organism: Homo sapiens
Morphology:lymphoblast


Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD11a +; CD19 +; CD20 +; CD28 -; CD38 +; CD49e +
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Cytogenetic Analysis:This is a hyperdiploid human cell line. The modal chromosome number was 48, occurring in 80% of cells, and the rate of polyploid cells was 3.4%. Three marker chromosomes are common to all cells. M1 and M2 [der(14)t(8;14) (q24;q32)] were probably derived from the balanced translocation between del(8) (p21.1;p21.3) and N14. The third marker, M3, is ?der(8)t(8;?) (p21.1;?). Most cells had three copies of N7. Both normal X and Y were clearly identifiable.
Age: 7
Gender: male
Ethnicity: Black, African
Comments:The cells are EBNA positive (EBNA +).
Although deposited with the ATCC as a plasmacytoma cell line, DNA fingerprinting has shown this line to be a derivative of Jiyoye (ATCC CCL-87 ), a Burkitt's lymphoma cell line.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.Maintain cell density between 3 X 10(5) and 3 X 10(6) viable cells/ml.Do not allow the cell density to exceed 4 X 10(6) cells/ml.
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CCL-87
References: 22894: Harris NS. Plasma cell surface antigen on human blood lymphocytes. Nature 250: 507-509, 1974. PubMed: 4549735
23445: . . J. Immunol. 118: 1480-1482, 1976.
24402: . . Proceedings of the Seventh Leukocyte Culture Conference : 15-28, 1973.
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375