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生長狀態(tài)：懸浮生長，少量貼壁
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
器官來源：盲腸
年限：56 years
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：1
物種來源：人
ATCC Number：CCL-254?
相關(guān)疾病：大腸癌
規(guī)格：0.1ml Designations: NCI-H498 [H498]
Depositors: ?AF Gazdar
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, with some loosely adherent cells
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: cecum
Disease: colorectal adenocarcinoma
Cellular Products:carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Rh+; CA19-9 antigen; TAG-72 antigen
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 9,11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 15
Cytogenetic Analysis:modal number = 51; range = 47 to 54.
This is a hyperdiploid human cell line. The modal chromosome number is 51, occurring in 42% of the cells. The rate of higher ploidies is 5%. The 2q+ is the only marker chromosome detected and common to all cells. There are four copies for N7; 3 copies each for N8, N13 and N22, and a single copy for the X.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1-2
Age: 56 years
Gender: male
Ethnicity: Caucasian
Comments:This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy.
The cells form glandular structures and secrete mucin.
The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA.
Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, the cells may be collected by centrifugation and then resuspended at 2 to 4 X 10(4) viable cells/ml. When the cell concentration becomes heavy with large clumps, aspirate well and and dilute approximately 1:10. If the clumps are allowed to get too large, most of the cells will die.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
23074: Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345