办公室疯狂高潮呻吟视频,又黄又大又爽又刺激的免费视频,激情综合一区二区三区,青草久久久国产线免费

769-P
    769-P
  • 平臺編號:bio-69561
  • 國際編號:CRL-1933?
  • 細(xì)胞信息: 769-P
  • 規(guī)格:Frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用及說明書:
    加載中……
  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))
ATCC Number:CRL-1933?
器官來源:腎臟
相關(guān)疾病:其他疾病
數(shù)量:大量
細(xì)胞形態(tài):上皮樣
運(yùn)輸方式:凍存運(yùn)輸
年限:63 years
生長狀態(tài):貼壁生長
是否是腫瘤細(xì)胞:1
物種來源:人
規(guī)格:0.1ml Designations: 769-P
Depositors: ?RD Williams
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: kidney
Disease: renal cell adenocarcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1975
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 10,14
D16S539: 9,13
D5S818: 12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,11
vWA: 18
Cytogenetic Analysis:This human cell line contained large numbers of tetra-, hexa-, and higher-ploid cells (2s populations). The modal (s) cell population which occurred in 32% of cells had the pseudodiploid karyotype, 46,XX,-3,-18,del(7) (q21.12;q22.3), ?t(3q?18q). The rate of 2s cells was 42%. Two marker chromosomes, del(7) (q21.12;q22.3) and ?t(3q?18q), were present in all s metaphases, but ?t(3q?18q) was absent in 2s cells. DMs were found in some s cells whereas they were seen in the majority of 2s cells. HSR chromosomes were not found. In s metaphases, both N3 and N18 had only single copy, and the X chromosome was paired.
Age: 63 years
Gender: female
Ethnicity: Caucasian
Comments:This line was derived from a primary clear cell adenocarcinoma.
The cells are globular with indistinct borders, have a high nucleus to cytoplasm ratio and exhibit both microvilli and desmosomes.
They can be grown in soft agar.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37?C.

      Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
      Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 35 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22648: Williams RD, et al. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528
22653: Williams RD, et al. In vitro cultivation of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102

固定電話:010-53515223

業(yè)務(wù)手機(jī):18610886853,18610241664,18701099593,18971386853,18701098095

微信號碼:vrmte521(18610886853企業(yè)微信);18610241664;biobw0905(18701098095)

企業(yè)公眾號:800185668

官方郵箱:biobw11@sina.com(微生物菌種查詢網(wǎng))

投訴電話:biobw8888(18701099593)