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生長狀態(tài)：懸浮生長
ATCC Number：CRL-2262?
相關(guān)疾病：其他疾病
年限：11 years
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
細(xì)胞類型：淋巴母細(xì)胞
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：100 ul Designations: SR
Depositors: ?M Beckwith
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast


Source: Disease: large cell immunoblastic lymphoma
Derived from metastatic site: pleural effusion
Cell Type: lymphoblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) -
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 14,15
D13S317: 11
D16S539: 11,13
D5S818: 11
D7S820: 9,12
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Age: 11 years
Gender: male
Ethnicity: Caucasian
Comments:SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages.
Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition.
SR cells have been reported to be Epstein-Barr virus genome negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723