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年限：Neonatal
數(shù)量：大量
運輸方式：凍存運輸
ATCC Number：PCS-200-010
細胞形態(tài)：其他
是否是腫瘤細胞：0
物種來源：人
生長狀態(tài)：貼壁生長
規(guī)格：0.2ml Name: Primary Epidermal Keratinocytes; Normal, Human, Neonatal
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Growth Properties: Adherent
Age: Neonatal
Morphology: Cobblestone appearance; cells are rounded, not flat
Volume per Vial: 1 ml
Gender: Male
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Primary Epidermal Keratinocytes; Normal, Human, Adult (ATCC PCS-200-011 )
Dermal Cell Basal Medium (ATCC PCS-200-030 )
Keratinocyte Growth Kit (ATCC PCS-200-040 )
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200 )
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 )
Trypsin Neutralizing Solution (ATCC PCS-999-004 )
Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 )
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 )
Phenol Red (ATCC PCS-999-001 )

Recomended Culture Conditions:See Product Information Sheet Comments:A complete solution to propagate neonatal keratinocytes. The serum-free media is formulated to inhibit fibroblast growth, and the low calcium concentration (60 μM) slows differentiation. No feeder layers, extracellular matrix proteins or other substrates are required.
Applications:Toxicology, wound repair, skin cancer, response to UV radiation, psoriasis, eczema, viral infection, gene delivery systems, cellular differentiation
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests: Bacteria and Yeast: Negative
Mycoplasma: Negative
Testing: Hepatitis B: Negative
Hepatitis C: Negative
HIV: Negative