年限:Adult
生長狀態(tài):貼壁生長
細胞形態(tài):其他
數(shù)量:大量
是否是腫瘤細胞:0
物種來源:人
運輸方式:凍存運輸
ATCC Number:PCS-100-011
規(guī)格:0.2ml Name: Primary Aortic Endothelial Cells; Normal, Human
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Aortic endothelium
Growth Properties: Adherent
Age: Adult
Morphology: Cobblestone appearance
Volume per Vial: 1 ml
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Vascular Cell Basal Medium (ATCC PCS-100-030 )
Endothelial Cell Growth Kit-BBE (ATCC PCS-100-040 )
Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041 )
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200 )
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 )
Trypsin Neutralizing Solution (ATCC PCS-999-004 )
Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 )
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 )
Phenol Red (ATCC PCS-999-001 )
Recomended Culture Conditions:See Product Information Sheet Comments:A complete solution to propagate Aortic Endothelial cells in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired.
Applications:Arteriolosclerosis, hypertension, arterial disease, vascular wound healing, inflammation, tissue remodeling, vascular differentiation, apoptosis, drug screening, stent development.
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests: Bacteria and Yeast: Negative
Mycoplasma: Negative
Testing: Hepatitis B: Negative
Hepatitis C: Negative
HIV-1: Negative