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L-M 拉丁名

(ATCC® CCL-1.2™) 統(tǒng)一編號(hào)

Organism: Mus musculus, mouse /

Organism Mus musculus, mouse

Product Format 提供形式 frozen

Morphology fibroblast

Culture Properties adherent

Biosafety Level 生物安全等級(jí) 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 100 days Gender male Strain C3H/An

Applications 用途 Tested and found negative for ectromelia virus (mousepox).

Clinical Data male Antigen Expression H-2k Genes Expressed H-2k Virus Resistance poliovirus 1, 2, 3 Comments Grows in protein free medium.

Tested and found negative for ectromelia virus (mousepox). Complete Growth Medium Medium 199 with Earle's BSS supplemented with 0.5% Bacto-peptone Subculturing

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: 2 to 3 times per week Subcultures are prepared by scraping.

Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.

Culture Conditions 培養(yǎng)條件

Temperature 培養(yǎng)溫度 : 37.0℃

Name of Depositor 寄存人 DJ Merchant

References 參考文獻(xiàn) Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534

Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205

Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756

Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385