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U-2 OS

(ATCC® HTB-96™)

Disease: osteosarcoma

Organism 微生物 Homo sapiens, human

Tissue bone

Product Format 提供形式  frozen

Morphology epithelial Culture Properties adherent

Biosafety Level  生物安全等級(jí) 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease osteosarcoma Age 15 years Gender female Ethnicity Caucasian

Applications This cell line is a suitable transfection host.

Karyotype Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range.

We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen.

Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::?), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others. Ref

Images Derivation J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Clinical Data 15 years Caucasian female J. Ponten and E.

Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Antigen Expression Antigen expression: Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) Receptor Expression

Receptor expression: insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II) Genes

Expressed osteosacoma derived growth factor (ODGF),Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)

Comments 注釋   Viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses.

Mycoplasma contamination was detected and eliminated in 1972.

Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007.

To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution.

Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution.

Allow the flask to sit at room temperature (or at 37℃) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Cryopreservation Culture medium, 95%; DMSO, 5%

Culture Conditions 培養(yǎng)條件 

Temperature 培養(yǎng)溫度 : 37℃

STR Profile Amelogenin: X CSF1PO: 13 D13S317: 13 D16S539: 11,12 D5S818: 11 D7S820: 11,12 THO1: 6,9.3 TPOX: 11,12 vWA: 14,18 Isoenzymes AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 2 PGM3, 1

Name of Depositor Hellstrom Year of Origin 1964

References 參考文獻(xiàn) Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080

Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590

Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487

Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590