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MIA PaCa-2

(ATCC® CRM-CRL-1420™) 編號

Organism 微生物 : Homo sapiens, human  / 

Cell Type 細胞類型 : epithelial cell (KRAS CRM)  /

Tissue: pancreas  / 

Disease: carcinoma

Organism Homo sapiens, human

Tissue pancreas

Cell Type epithelial cell (KRAS CRM)

Product Format 提供形式 frozen

Morphology attached epithelial with floating rounded cells

Culture Properties adherent, single cells and loosely attached clusters

Biosafety Level 生物安全等級1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease carcinoma Age 65 years Gender Male Ethnicity Caucasian

Applications 用途 For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009
Intended Use

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

Storage Conditions 保藏方法 liquid nitrogen vapor phase

Karyotype

This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.

Derivation The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.

Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).

Clinical Data 65 years Caucasian male

Genes Expressed human colony stimulating factor, subclass I (CSF-I); plasminogen activator
Cellular Products human colony stimulating factor, subclass I (CSF-I); plasminogen activator
CommentsCertificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
 
Certified Reference Material produced under an ISO Guide 34:2009 accredited process.
Item has been tested for KRAS mutation (p.G12C c.34G>T)
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.
 
The line is sensitive to asparaginase. Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.

Subculturing

Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37℃) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze Medium: Complete growth medium 95%; DMSO, 5%

Storage Temperature 保藏溫度 : Liquid nitrogen vapor phase
Culture Conditions 培養(yǎng)條件

Temperature 培養(yǎng)溫度 : 37℃STR Profile

Amelogenin: X

CSF1PO: 10

D13S317: 12,13

D16S539: 10,13

D5S818: 12,13

D7S820: 12,13

THO1: 9,10

TPOX: 9

vWA: 15
Isoenzymes G6PD, B

Name of Depositor A Yunis

Year of Origin 1975

References 參考文獻 Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590

 Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740

 Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455

 Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918