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細(xì)胞CELL>
示蹤細(xì)胞(GFP/LUC/RFP...)>
人結(jié)腸癌細(xì)胞(熒光素酶標(biāo)記)(DMEM) 名稱SW620/LUC(人結(jié)腸癌細(xì)胞(熒光素酶標(biāo)記))(DMEM)(STR鑒定正確)
種屬人類
年齡(性別)男性,51歲
組織來源結(jié)直腸腺癌,來自轉(zhuǎn)移淋巴結(jié)
生長特性貼壁細(xì)胞
細(xì)胞形態(tài)上皮細(xì)胞樣
生物安全等級1
生長培養(yǎng)基DMEM+10%FBS+1%P/S
推薦傳代比例1:3-1:4
推薦換液頻率2~3次/周
倍增時間~20-26小時
凍存條件
凍存液:55%基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO
溫度:液氮
培養(yǎng)條件
氣相:空氣,95%;CO2,5%
溫度:37℃
致瘤性Yes,in nude mice(Tumors developed within 21 days at 100%frequency(5/5)in nude mice inoculated subcutaneously with 1×10^7 cells).
抗原表達情況Blood Type A;Rh+
基因表達情況Carcinoembryonic antigen(CEA)0.15 ng/10^6 cells/10 days;transforming growth factor alpha;matrilysin.The cells are negative for expression of CSAp(CSAp-)and colon antigen 3,negative.The cells are positive for keratin by immunoperoxidase staining.The line is positive for expression of c-myc,K-ras,H-ras,N-ras,Myb,sis and fos oncogenes.
保藏機構(gòu)ATCC;CCL-227 ECACC;87051203
固定電話:010-53515223
業(yè)務(wù)手機:18610886853,18610241664,18701099593,18971386853,18701098095
微信號碼:vrmte521(18610886853企業(yè)微信);18610241664;biobw0905(18701098095)
企業(yè)公眾號:800185668
官方郵箱:biobw11@sina.com(微生物菌種查詢網(wǎng))
投訴電話:biobw8888(18701099593)