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THP-1 

(ATCC? TIB-202?)

Organism Homo sapiens, human 

Tissue peripheral blood Cell 

Type monocyte 

Product Format frozen 

Morphology monocyte 

Culture Properties suspension 

Biosafety Level 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Disease acute monocytic leukemia 

Age 1 year infant 

Gender male 

Applications These cells are suitable as a transfection host. 

Storage Conditions liquid nitrogen vapor phase 

Images 

Antigen Expression HLA A2, A9, B5, DRw1, DRw2 Ref Receptor Expression complement (C3), expressed Ref Fc, expressed Genes Expressed lysozyme,HLA A2, A9, B5, DRw1, DRw2 Cellular Products lysozyme Comments The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin. Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). 

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. 

To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%. 

Subculturing Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. 

Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 x 105 viable cells/mL. 

Subculture when cell concentration reaches 8x105 cells/mL.Do not allow the cell concentration to exceed 1 x 106 cells/mL. Corning? T-75 flasks (catalog #431464) are recommended for subculturing this product. 

Medium Renewal: Every 2 to 3 days 

Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5% 

Temperature: 37.0°C 

STR Profile Amelogenin: X,Y 

CSF1PO: 11,13 

D13S317: 13 

D16S539: 11,12 

D5S818: 11,12 

D7S820: 10 

THO1: 8,9.3 

TPOX: 8,11 

vWA: 16

Name of Depositor S Tsuchiya 

References Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641 

Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543 

Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475 Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 

Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116 

Hsu HY, et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819 

Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812 

Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. PubMed: 8703011 

Ollivier V, et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996. PubMed: 8702838 

Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer 26: 171-176, 1980. PubMed: 6970727 

Cross References Nucleotide (GenBank) : X70326 H.sapiens MacMarcks mRNA.