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Bacteria Collection: Bacteroides vulgatus
NCTC Number: NCTC 11154
Current Name: Bacteroides vulgatus
Original Strain Reference: ATCC 8482
Other Collection No: ATCC 8482
Previous Catalogue Name: Bacteroides vulgatus
Type Strain: Yes
Family: Bacteroidaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 24-48 hours, 37°C, anaerobic
Conditions for growth on liquid media: bhi agar(bmrl formula),bhi broth(bmrl formula),37, strict anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1273874
16S rRNA Gene Sequence: >gb|AF176693|ATCC8482|Bacteroides vulgatus 16S-23S rDNA intergenic spacer region.| ggagaggacgaaaga... >gb|AJ867050|ATCC 8482|Bacteroides vulgatus 16S rRNA gene, strain ATCC 8482.| caaactaggaccgag...
23S rRNA Gene Sequence: >gb|AF176693|ATCC8482|Bacteroides vulgatus 16S-23S rDNA intergenic spacer region.| ggagaggacgaaaga... >gb|AY155596|ATCC 8482|Bacteroides vulgatus 23S ribosomal RNA gene, partial sequence.| tagtcgaggagtcgg...
Bibliography: EGGERTH A H & GAGNON B H 1933 J BACT 25 389; CATO E P & JOHNSON J L 1976
Extended Bibliography: showhide Show bibliography
Ref #: 25269
Author(s): Liu,C.;Song,Y.;McTeague,M.;Vu,A.W.;Wexler,H.;Finegold,S.M.
Journal: FEMS Microbiol Lett
Title: Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers
Volume: 222
Page(s): 9-16
Year: 2003
Keyword(s): Bacteriological Techniques Bacteroides fragilis/*classification/*genetics/growth & development DNA Transposable Elements/genetics Phenotype Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics
Remarks: We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were first divided into three subgroups by multiplex PCR-G, followed by three multiplex PCR assays with three species-specific primer mixtures for identification to the species level. The primers were designed from nucleotide sequences of the 16S rRNA, the 16S-23S rRNA intergenic spacer region and part of the 23S rRNA gene. The established two-step multiplex PCR identification scheme was applied to the identification of 155 clinical isolates of the B. fragilis group that were previously identified to the species level by phenotypic tests. The new scheme was more accurate than phenotypic identification, which was accurate only 84.5% of the time. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species. This will permit more accurate assessment of the role of various B. fragilis group members in infections and of the degree of antimicrobial resistance in each of the group members.
URL: 12757940
Data: Type strain / ATCC in 1977 / A.H. Eggerth in 1942 (Redeposited in 1954) / Eggerth, A. H. & Gagnon, B. H. (1933) J. Bact. 25, 389 / Moore, W. E. C. & Holdemann, L. V. (1973) Int. J. syst. Bact. 23, 69 / Cato, E. P. & Johnson, J. L. (1976) Int. J. syst. Bact. 26, 230
Accession Date: 01/01/1977
History: ISOLATED BY EGGERTH A H,1942;REPLACEMENT IN 1954
Authority: Eggerth and Gagnon 1933 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S508 (Bacteroides vulgatus Eggerth and Gagnon 1933) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country