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　　　Species:Escherichia coli

Group:B

Strain designation:BL21(DE3)

Accession number:LMBP 1455

Depositor:Prof.Dr W.Studier1

1 Biology Department,Brookhaven National Laboratory,Upton,USA

Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

Medium:LB-Lennox

Selection marker:/

Temperature:28°C

Genotype:F(-)ompT gal dcm lon hsdSB(rB-mB-)λ(DE3[lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Phenotype:-

Properties:The strain contains DE3,aλprophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq.IPTG is required to induce expression of the T7 RNA polymerase.Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs.The strain does not contain the lon protease and is also deficient in the outer membrane protease,OmpT.The lack of two key proteases reduces the degradation of heterologous proteins expressed in the strain.

Further information:The full genome sequence of this strain is available at the ENA/GenBank nucleotide sequence databases under accession number CP001509.3.

Original reference:Studier and Moffatt,J.Mol.Biol.189(1986),113-130[PMID:3537305][DOI:10.1016/0022-2836(86)90385-2]

Related reference:Daegelen et al.,J.Mol.Biol.394(2009),634-643[PMID:19765591][DOI:10.1016/j.jmb.2009.09.022]

Jeong et al.,J.Mol.Biol.394(2009),644-652[PMID:19786035][DOI:10.1016/j.jmb.2009.09.052]

Studier et al.,J.Mol.Biol.394(2009),653-680[PMID:19765592][DOI:10.1016/j.jmb.2009.09.021]

Restricted use:-BCCM MTA

Refer in your Materials and Methods:

Escherichia coli B BL21(DE3)(LMBP 1455)is available at BCCM/GeneCorner.It was deposited by Prof.Dr W.Studier and was published in Studier and Moffatt,1986.