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Staphylococcus aureus subsp. aureus Rosenbach  拉丁名

ATCC 700699 ATCC編號(hào)

Deposited As    Staphylococcus aureus Rosenbach

Strain Designations  菌株別名 Mu50 [NRS1]

Application 用途 Drug discovery

Isolation 分離原 Isolated from pus and debrided tissue at surgical incision in sternum of 4 month-old infant, Japan, 1996.

Biosafety Level  安全等級(jí) 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 Freeze-Dried

Storage Conditions 保存條件

Frozen 冷凍物 : -80°C or colder

Freeze-Dried 凍干物 : 2°C to 8°C

Live Culture 活菌 : See Propagation Section

Preceptrol no

Genotype    pvl negative 

Genome Sequenced Strain 

Staphylococcus aureus subsp. aureus Mu50 DNA, complete genome

Type Strain    no

Antibiotic Resistance    Methicillin resistant

Oxacillin resistant

Reduced vancomycin susceptibility

Comments 

Degree of resistance tends to decrease depending on the culture condition. Recommend propagating strain in Brain Heart Infusion with 4 mg/L vancomycin (ATCC Medium 2174).

Genome sequenced strain

MRSA

SCCmec: Type II

spa type Ridom: t002

spa type Kreiswirth: TJMBMDMGMK

pvl gene amplification: Negative

Phenotypic and genotypic information for ATCC MRSA strains, such as SCCmec, PFGE, or spa typing, is provided for informational purposes only. This information was compiled from testing performed by one or more sources, including ATCC, strain depositor, published data, or contracted research.

Medium    ATCC Medium 2174: Brain heart infusion medium with vancomycin 4 mcg/ml

Growth Conditions 

Temperature: 37°C

Atmosphere: Aerobic

Genotype    pvl negative

Name of Depositor    K Hiramatsu

Special Collection    NCRR Contract

Isolation 

Isolated from pus and debrided tissue at surgical incision in sternum of 4 month-old infant, Japan, 1996.

References 

Hiramatsu K, et al. Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet 350: 1670-1673, 1997. PubMed: 9400512

Hiramatsu K, et al. Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. J. Antimicrob. Chemother. 40: 135-136, 1997. PubMed: 9249217

Keiichi Hiramatsu, personal communication

Monecke S, et al. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One 6(4): e17936, 2011. PubMed: 21494333

Cross References 

Nucleotide (GenBank) : BA000017 Staphylococcus aureus subsp. aureus Mu50 DNA, complete genome