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Klebsiella quasipneumoniae Brisse et al L拉丁名

(ATCC700603) 統(tǒng)一編號

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Klebsiella quasipneumoniae strain K6  is a whole-genome sequenced bacterium that was isolated from the urine of a hospitalized patient in Richmond, Virginia. This bacterium produces beta-lactamase SHV-18 and can be used as a control for extended-spectrum beta-lactamase production. This organism is a CLSI quality control strain for antimicrobial susceptibility testing.

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Strain Designations 菌種別名: K6 [CCUG 45421, LMG 20218, MCV37]  / 

Type Strain 模式菌種: no  / 

Biosafety Level 生物安全等級 : 2

Deposited As Klebsiella pneumoniae (Schroeter) Trevisan

Application 用途Produces beta-lactamase SHV-18

Quality control strain

Resistant to ampicillin

Resistant to aztreonam

Resistant to cefoxitin

Resistant to cefpodoxime

Resistant to ceftazidime

Resistant to chloramphenicol

Resistant to piperacillin

Resistant to tetracycline

Respiratory research

Sensitive to amoxicillin-clavulanate

Sensitive to cefepime

Intermediate resistance for cefotaxime

Sensitive to ciprofloxacin

Sensitive to imipenem

Sensitive to piperacillin-tazobactam

Intermediate resistance for Tobramycin

Sensitive to trimethoprim-sulfamethoxazole

Susceptibility disc testing

Susceptibility testing

Control for extended-spectrum beta-lactamase production
Isolation Urine from hospitalized patient, Richmond, VA, 1994

Product Format 提供形式 freeze-dried

Storage Conditions保藏方法

Frozen（冷凍物）: -80℃ or colder
Freeze-Dried（凍干物）: 2℃ to 8℃
Live Culture（活物）: See Propagation Section 
Preceptrol? no

Type Strain 模式菌種no 

Antibiotic ResistanceResistant to cefoxitin
Resistant to cefpodoxime
Resistant to ceftazidime
Resistant to chloramphenicol
Resistant to piperacillin
Resistant to ceftriaxone
Sensitive to trimethoprim-sulfamethoxazole
Sensitive to imipenem
Resistant to aztreonam
Resistant to ampicillin
Resistant to tetracycline
Sensitive to amoxicillin-clavulanate
Sensitive to cefepime
Sensitive to cefotaxime
Sensitive to ciprofloxacin
Sensitive to piperacillin-tazobactam
Intermediate resistance to gentamicin
Comments MIC (?g/mL):  Ampicillin >64 Amoxicillin-clavulanate 8
Aztreonam 64 Aztreonam-clavulanate <=0.25
Cefotaxime 8  Cefotaxime-clavulanate <=0.25
Cefpodoxime 16 Cefpodoxime-clavulanate 1 Ceftazidime 32
Ceftazidime-clavulanate 1
Ceftriaxone 16
Ceftriaxone-clavulanate <=0.25
Cefepime 1
Cefoxitin 32
Chloramphenicol >32
Ciprofloxacin 0.5
Gentamicin 8
Imipenem <=1
Piperacillin >128
Piperacillin-tazobactam 16 Tetracycline 16 Tobramycin 4

Trimethoprim-sulfamethoxazole

Medium 培養(yǎng)基 ATCC? Medium 3: Nutrient agar or nutrient broth

Growth Conditions生長條件

Temperature 培養(yǎng)溫度: 37℃

Atmosphere 需氧情況 : Aerobic

Name of Depositor 寄存人 JM Swenson

Special Collection NCRR

ContractChain of Custody ATCC <-- JM Swenson <-- MJ Ferraro <-- G Jacoby <-- H Dalton
Isolation Urine from hospitalized patient, Richmond, VA, 1994
References
Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

Rasheed JK, et al. Characterization of the extended-spectrum beta-lactamase reference strain, Klebsiella pneumoniae K6 (ATCC 700603), which produces the novel enzyme SHV-18. Antimicrob. Agents Chemother. 44: 2382-2388, 2000. PubMed: 10952583

 Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

BD Phoenix Gram Negative AST NMIC/ID QC Set. BD Becton Dickinson; Phoenix

MicroScan Dried Gram Negative Panel QC Set. Dade/Siemens.

Sensititre? MIC Susceptibility. Thermo Scientific? Sensititre? Systems, Trek Diagnostic Systems.

Jana M Swenson, personal communication

VITEK 2 AST-GN QC Set. bioMerieux.

MicroScan ESBL plus QC Set. Dade/Siemens.

MicroScan Synergies plus Gram Negative QC Set. Dade/Siemens.

ETEST ESBL STRIP. bioMerieux.

Cross References

Nucleotide (GenBank) : AX111170 Sequence 1903 from Patent WO0123604.

Nucleotide (GenBank) : AF132290 Klebsiella pneumoniae plasmid-encoded extended-spectrum beta-lactamase (bla SHV-18) gene, complete cds.