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Dermal Cell Basal Medium
PCS-200-030?

Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions? system. 

This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.  

To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation. 

To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.
Optional media supplements:

Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
Phenol Red (ATCC PCS-999-001)
 

Product category
Media
Applications
Cell growth and viability
Volume
485 mL
Storage conditions
2°C to 8°C
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Documentation

Product sheet
Certificate of analysis
Safety data sheet
Biosafety Icon BSL 1
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
Specific applications
Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions? system.

This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.  

To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation. 

To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.
Optional media supplements:

Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
Phenol Red (ATCC PCS-999-001)
 

Handling information
Handling procedure
Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
Obtain one bottle of Dermal Cell Basal Medium (485 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer
Table 1. If using the Keratinocyte Growth Kit (ATCC? PCS-200-040), add the indicated volume for each component: