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Candida albicans (Robin) Berkhout (ATCC? 90028?)

Strain Designations: NCCLS 11  /  Product Format: freeze-dried

Deposited As

Candida albicans (Robin) Berkhout, anamorph

Strain Designations

NCCLS 11

Application

Produces collagenase

Susceptibility disc testing

Susceptibility testing

Reference strain for Clinical and Laboratory Standards Institute(CLSI)-developed Antifungal Susceptibility Testing.

Biosafety Level 1

Product Format freeze-dried

Storage Conditions

Frozen: -80℃ or colder
Freeze-Dried: 2℃ to 8℃
Live Culture: See Propagation Section

Type Strain no 

Preceptrol? yes

Comments

Effect of amphotericin B on vitality

Fluconazole-sensitive

Multilocus genotyping

For additional infromation, see CLSI M27-A2 (Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standards-Second Edition).

Morphology

After 2 days on YM agar at 30℃, colonies white, smooth, butyrous. Cells globose to ovoidal, single or in pairs, pseudohyphae not observed.

Medium

ATCC? Medium 200: YM agar or YM broth
ATCC? Medium 1245: YEPD
ATCC? Medium 28: Emmons' modification of Sabouraud's agar

 Growth Conditions

Temperature: 30℃ to 35℃

Atmosphere: Typical aerobic

Squenced Data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
 AAGGATCATTACTGATTTGCTTAATTGCACCACATGTGTTTTTCTTTGAAACAAACTTGCTTTGGCGGTGGGCCCAGCCTGCCGCCAGAGGTCTAAACTTACAACCAATTTTTTATCAACTTGTCACACCAGATTATTACTAATAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATATGAATTGCAGATATTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTCCCTCAAACCGCTGGGTTTGGTGTTGAGCAATACGACTTGGGTTTGCTTGAAAGACGGTAGTGGTAAGGCGGGATCGCTTTGACAATGGCTTAGGTCTAACCAAAAACATTGCTTGCGGCGGTAACGTCCACCACGTATATCTTCAAACTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAAG

 D1D2 region of the 28S ribosomal RNA gene
 GCGACTTAAGATCATTATGCCAACATCCTAGGTAAAAACCGCAGTCCTCGGTCTAGGCTGGCAGTATCGTCAGAGGCTATAACACACAGCAGAAGCCGTGCCACATTCCTCCGCCATTATCCTGCCGCTCCAAACCGATGCTGGCCCGGTAAACCGCAGCGGCCGCCCCCGAGAGAGCAGCATGCAAAATACCAAGTCTGATCTCAAGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAACTTTACACAGACCCGGGTCATCTCATCGCACGGGATTCTCACCCTCTGTGACGTCCTGTTCCAAGGAACATAGACAAGAGCCGGGCCCAAAGATACCTTCTTCAAATTACAACTCGGACGCCAAAGACGCCAGATTTCAAATTTGAGCTTTTGCCGCTTCACTCGCCGCTACTGAGGCAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTGATATGC

Morphology

After 2 days on YM agar at 30℃, colonies white, smooth, butyrous. Cells globose to ovoidal, single or in pairs, pseudohyphae not observed.

Name of Depositor

MA Pfaller

Isolation

Blood, Iowa

Cross References

Nucleotide (GenBank) : AX109678 Sequence 411 from Patent WO0123604.

Nucleotide (GenBank) : AB006854 Candida albicans gene for CYP51 variant1, complete cds.

References

Espinel-Ingroff A, et al. Collaborative comparison of broth macrodilution and microdilution antifungal susceptibility tests. J Clin Microbiol 30: 3138-3145, 1992. PubMed: 1452697

 Pfaller MA, et al. Selection of candidate quality control isolates and tentative quality control ranges for in vitro susceptibility testing of yeast isolates by National Committee for Clinical Laboratory Standards proposed standard methods. J Clin Microbiol 32: 1650-1653, 1994. PubMed: 7929752

 Espinel-Ingroff A, et al. Comparison of two alternative microdilution procedures with the National Committee for Clinical Laboratory Standards reference macrodilution method M27-P for in vitro testing of fluconazole-resistant and -susceptible isolates of Candida albicans. J Clin Microbiol 33: 3154-3158, 1995. PubMed: 8586692

 Liao RS, et al. Assessment of the effect of amphotericin B on the vitality of Candida albicans. Antimicrob. Agents Chemother. 43: 1034-1041, 1999. PubMed: 10223911

 Luu LN, et al. Multilocus genotyping indicates that the ability to invade the bloodstream is widespread among Candida albicans isolates. J. Clin. Microbiol. 39: 1657-1660, 2001. PubMed: 11283111

 Yang HC, et al. Extrusion of fluorescein diacetate by multidrug-resistant Candida albicans. Mycoses 44: 368-374, 2001. PubMed: 11766100

 Hatano K, et al. Antifungal mechanism of FK463 against Candida albicans and Aspergillus fumigatus. J. Antibiot. 55: 219-222, 2002. PubMed: 12003006

 Nishimura M, et al. Cell-associated collagenolytic activity by Candida albicans. Mycopathologia 153: 125-128, 2001.

Method for Antifungal Disk Diffusion Susceptibility Testing of Yeast; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M44-A2.

Medical microbiology--Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 84: Microdilution; Special requirements for testing of fungi against antifungal agents. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-84: 2002, 2002