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Escherichia coli (Migula) Castellani and Chalmers 拉丁名

(ATCC 8739) 統(tǒng)一編號

Classification 類別 Enterobacteriaceae, Escherichia

Strain Designations 菌株別名 Crooks

Application 用途 Assay of antimicrobial preservatives；

Bioresistance testing；

Efficacy testing；

Media testing；

Preparatory test control；

Quality control strain；

Testing；

Testing antimicrobial handwashing formulations；

Reduces dehydroascorbic acid；

Food testing；

Pharmaceutical and Personal Care；

Quality control strain for Biosynth and Difco products；
Isolation 分離基物 Feces

Type Strain 模式菌株 no

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
 Product Format 提供形式 freeze-dried

Storage Conditions 保藏條件

Frozen 冷凍物: -80℃ or colder
Freeze-Dried 凍干物: 2℃ to 8℃
Live Culture 活物: See Propagation Section

Preceptrol no

Genome Sequenced Strain

Escherichia coli ATCC 8739, complete genome
Type Strain 模式菌株 no

Comments 注釋 Genome sequenced strain

Customers must use Nutrient broth to initiate this culture rather than Tryptic Soy. This strain does not grow on TSB (although many other E. coli will). The medium used successfully in our labs is Becton Dickinson Nutrient Broth (catalog number 234000) and Nutrient Agar (catalog number 213000).

Medium 培養(yǎng)基 ATCC  Medium 3: Nutrient agar or nutrient broth

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 37℃

Atmosphere 需氧情況 : Aerobic

Name of Depositor 寄存人 IC Gunsalus

Chain of Custody 來源國家 ATCC <-- IC Gunsalus <-- GC Crooks

Isolation 分離基物 Feces

References 參考文獻(xiàn) ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

J. Biol. Chem. 141: 853, 1941.

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 2003

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (total viable aerobic count). Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.12, 1997

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (tests for specified micro-organisms). Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.13, 1997

European Pharmacopoeia Commission Efficacy of antimicrobial preservation. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.3, 1997

U.S. Pharmacopeia General Chapters:ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

U.S. Pharmacopeia Dietary Supplement Chapters:Microbial Enumeration Tests-Nutritional and Dietary Supplements. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

Jones CB, Platt JH. Propofol composition containing edetate. US Patent 5,714,520 dated Feb 3 1998

Fendler EJ, et al. Antimicrobial cleansing composition containing chlorhexidine, an amphoteric surfactant, and an alkyl polyglucoside. US Patent 5,719,113 dated Feb 17 1998

Applied Biosystems, MicroSeq Pharmaceutical Validation Panel.

Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.

Microbial limit test. Tokyo, Japan:Japanese Pharmacopoeia;JP Jp14e.part I.35.

Microbial limit test for crude drugs. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.36.

Preservatives-effectiveness test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.12.

Food microbiology. Method 12.3: Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphyloccus aureus and other species)--Detection and MPN technique for low numbers. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 5013.12.3:2004.

Antimicrobial products - Test for antimicrobial activity and efficacy. Tokyo, Japan:Japanese Standards Association;JIS Z 2801, 2000

Microbiology of food and animal feeding stuffs-- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)--Part3: Detection and MPN technique for low numbers. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 6888-3:2003.

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Ophthalmic optics--Contact lens care products--Antimicrobial preservative efficacy testing and guidance on determining discard date. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14730:2000.

Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2275-03E01.

Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria. London, UK:British Standards Institution;British Standard BS ISO 21149:2006.

Cosmetics --- Microbiology --- Detection of Escherichia coli. London, UK:British Standards Institution;British Standard BS ISO 21150:2006.

Microbiology of food and animal feeding stuffs --- Horizontal method for the determination of low numbers of presumptive Bacillus cereus --- Most probable number technique and detection method. London, UK:British Standards Institution;British Standard BS EN ISO 21871:2006.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

U.S. Pharmacopeia General Chapters:Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. Rockville, MD USP34-NF29, 2011

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Cross References

Nucleotide (GenBank) : CP000946 Escherichia coli ATCC 8739, complete genome

JGI Project ID : 4002730 Escherichia coli C str. ATCC 8739 finished genome