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Pseudomonas aeruginosa (Schroeter) Migula 菌株拉丁名

 (ATCC? 47085?) 菌株編號(hào)

Strain Designations 菌株別名 PAO1-LAC

Application 用途 Transformation host Opportunistic pathogen research

Biosafety Level 生物安全等級(jí) 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏方法

Frozen 冷凍物 : -80℃ or colder

Freeze-Dried 凍干物 : 2℃ to 8℃

Live Culture 活菌 : See Propagation Section

Preceptrol? no

Genotype lacIq+ delta(lacZ)M15+ tetA+ tetR+

Genome Sequenced Strain Pseudomonas aeruginosa PAO1, complete genome

Type Strain 模式菌株 no

Comments 注釋 For use with the expression vector pUCP19 (ATCC 87110). Phenotype verified: tetR.

Constructed by stable integration of a mini-D3112 transposable element containing sequences allowing lacZalpha complementation.

Purified genomic DNA from this organism is available as ATCC 47085D-5 Genome sequenced strain

Medium 培養(yǎng)基 ATCC? Medium 1631: LB medium (ATCC medium 1065) with 10 mcg/ml tetracycline

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度 : 30℃

Atmosphere 需氧情況 : Aerobic Genotype lacIq+ delta(lacZ)M15+ tetA+ tetR+

Name of Depositor 寄存人 RR Karkhoff-Schweizer

Special Collection NCRR Contract

References 參考文獻(xiàn) Karkhoff-Schweizer RR, Schweizer HP. Utilization of a mini-Dlac transposable element to create an alpha-complementation and regulated expression system for cloning in Pseudomonas aeruginosa. Gene 140: 7-15, 1994. PubMed: 8125342

Stover CK, et al. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406: 959-964, 2000. PubMed: 10984043

Cross References Nucleotide (GenBank) : AE004091 Pseudomonas aeruginosa PAO1, complete genome