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Escherichia coli (Migula) Castellani and Chalmers 拉丁名 

(ATCC? 47076?) 菌株編號

Classification Enterobacteriaceae, Escherichia 

Strain Designations 菌株別名 MG1655 

Application 用途 Transformation host Bacteriophage host 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 

Shipping Information Frozen gycerol stock of E. coli 

Storage Conditions 保藏條件 -80°C or colder 

Preceptrol? no 

Genotype F- lambda- Type 

Strain no 

Comments 注釋 Electronic access to the correlations between the restriction map and the ordered phage library of Kohara are available over the Internet by anonymous ftp at these hosts: ncbi.nlm.nih.gov and utmmg.med.uth.tmc.edu. 

Host strain with well-characterized restriction maps for NotI, SfiI, XbaI and BlnI. Can be used as a molecular size marker for pulsed-field gel electrophoresis. 

AE000111 through AE000510 are individual GenBank numbers for the complete sequence, U00096 Derived from the parent strain W1485 by curing of the F plasmid. 

Medium 培養(yǎng)基 ATCC? Medium 1065: LB Agar/Broth, Miller 

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 37°C 

Atmosphere 需氧情況 : Aerobic 

Genotype F- lambda- 

Name of Depositor 寄存人 GM Weinstock 

Chain of Custody 來源國家 ATCC <-- GM Weinstock <-- MG1655 

References 參考文獻 Blattner FR. Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 21: 5408-5417, 1993. PubMed: 8265357 

Blattner FR, et al. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462, 1997. PubMed: 9278503

Dicker IB. Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J. Bacteriol. 173: 334-344, 1991. PubMed: 1987124 

Heath JD, et al. NotI genomic cleavage map of Escherichia coli K-12 strain MG1655. J. Bacteriol. 174: 558-567, 1992. PubMed: 1729244 

Plunkett G III, et al. Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 21: 3391-3398, 1993. PubMed: 8346018 

Jensen KF. The Escherichia coli K-12 'wild types' W3110 and MG1655 have an rphframeshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. Gene 175: 3401-3407, 1993. PubMed: 8501045 

Freddolino PL, Amini S, and S Tavazoie. Newly Identified Genetic Variations in Common Escherichia coli MG1655 Stock Cultures. J. Bacteriol. 194(2): 303-306, 2012. PubMed: 22081388 

Cross References Nucleotide (GenBank) : U00096 complete sequence 

Nucleotide (GenBank) : X72920 intercistronic region 

Nucleotide (GenBank) : X64592 gene for ribosomal protein S7, partial 

Nucleotide (GenBank) : L10328 chromosomal region from 81.5 to 84.5 minutes 

Nucleotide (GenBank) : L19201 chromosomal region from 87.2 to 89.2 minutes 

Nucleotide (GenBank) : M87049 chromosomal region from 84.5 to 86.5 minutes 

Nucleotide (GenBank) : U00006 chromosomal region from 89.2 to 92.8 minutes 

Nucleotide (GenBank) : X54797 firA gene, firA(200) and firA(201) allele, and skp gene (3' end)